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1. SPECTRAL SLIT WIDTH (if applicable)
When using an instrument on which the slit-width is variable at the selected wavelength, the
slit-width must be small compared with the half-width of the absorption band but it must be
as large as possible to obtain a high value of I0. Therefore, a slit-width is chosen such that
further reduction does not result in a change in absorbance reading.
Method and Limits:
1. Switch the system on and start the Scan module.
2. Select SETUP and set the following parameters:
X Mode = Nanometres
Start wavelength = 660.0 nm
Stop wavelength = 650.0 nm
Y Mode = % T
Scan rate = 10 nm/min
Gain = (100) see 4 below
PA/PH/OMCL (07) 11 DEF CORR - OMCL Guideline on Qualification of UV-Visible spectrophotometers (Annex 3)